ABSTRACT
Aim To investigate the expression of CAR-MA3, NF-κB in hepatocellular carcinoma tissues and the underlying mechanism of sodium aescinate in inhib-iting the proliferation of human hepatocellular carcino-ma cells. Methods The expression of CARMA3 and NF-κB in HCC tissues were detected by tissue microar-ray immunohistochemistry. MTT was used to determine the effect of sodium aescinate on the proliferation of HCC cells. Cell apoptosis was detected by flow cytom-etry. The expression of CARMA3, NF-κB protein in HepG2 and Hep3B cells treated with sodium aescinate was detected by Western blot and cell immunofluores-cence. Results Tissue microarray analysis showed that the expression of CARMA3 in HCC was up-regulated compared with the adjacent adjacent liver tissues, and the histopathological differentiation, TNM stage, tumor volume and prognosis were correlated. Sodium aesci-nate in 40 μmol·L-1concentration ( IC50) inhibited the growth of HCC cell lines, promoting its apoptosis, but without toxic effects on normal liver cells. Western blot and cell immunofluorescence detection of sodium aescinate could significantly inhibit the expression of CARMA3 and NF-κB. Conclusion Sodium aescinate can effectively inhibit the proliferation of HCC cells by inhibiting the activation of CARMA3/NF-κB signaling in HCC.
ABSTRACT
The origin and distribution of oligosaccharides of TCM are extensive, and its special biological activity and chemical properties have great potential in health preserving and disease prevention and treatment. The researches about its components are increasing, and its research and application are expanded. Oligosaccharides of TCM can be obtained by natural extraction, degradation and artificial synthesis. Among them, the natural extraction method is the most suitable for chromatography. The oligosaccharides of TCM have different groups and connections, and the structure is complex, with different chemical reactions in the process. This article reviewed the research overview of oligosaccharides of TCM from the aspects of separation, biological activity and chemical reactions in the process.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the frequencies distribution of GSTT1 and GSTM1 null genotypes in 24 Yunnan populations.</p><p><b>METHODS</b>GSTT1 and GSTM1 genotypes were analyzed by PCR procedure.</p><p><b>RESULTS</b>The range of frequencies for GSTT1 and GSTM1 null genotypes in the populations were 0.188-0.633 and 0.400-0.745, respectively, and in the districts were 0.286-0.583, 0.433-0.745 respectively. There was significant relationship between GSTT1 frequencies and populations.</p><p><b>CONCLUSION</b>The frequencies of GSTT1 and GSTM1 null genotypes in 24 Yunnan populations were different, but they were almost the same in different districts of Yunnan.</p>